CMarZ Barcoding Association

Protocols

Samples

Policy on Use of Archived Samples   CMarZ samples may be examined by interested researchers and students with these guidelines.

Sample Collection   How to collect and preserve zooplankton for optimal success in genetic analyses.

In the Lab

CMarZ mtCOI Barcoding Protocol    Our procedure for extracting and sequencing the MtCOI gene.

Protocol for DNA extraction, amplification and sequencing of Zooplankton

Protocol for DNA extraction and purification for Copepods

Total DNA Extraction from small metazoans. Modified from Schizas et al. (1997)

DNeasy® Blood & Tissue Handbook: DNeasy Blood & Tissue Kit, DNeasy 96 Blood & Tissue Kit - for purification of total DNA.

Data

Policy on DNA Barcode Data    Requirements for submission of DNA sequences to GenBank; agreement between person providing specimens and the DNA lab on publishing data; use of voucher material.

Specimen Barcode Metadata guidelines - we request a tab-separated flat file, exported from XLS using the this template, to Bob Groman at WHOI.